APPLICATION
Application Note
Measured by the ablility to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well place coated with 500ng/ml anti-CD3 and 10 μg/ml human B7H4/Fc. The presence of human B7H4/Fc at 10 μg/ml inhibited anti CD3 response by 30-50%. Optimal dilutions should be determined by each laboratory for each application.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 100 μl ddH₂O. Lyophilized from 0.2μm-filtered PBS.
Preservative
No preservative
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.
Region/Sequence
The extracellular domain of human B7-H4 (a.a. 29-258) is fused to the N-terminus of the Fc region of human IgG1.
Expression System
CHO cells
Purity
??98% by SDS-PAGE.
Endotoxin
< 0.06 EU/μg
Conjugation
Unconjugated
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
V-set domain containing T cell activation inhibitor 1 , B7-H4 , B7H4 , B7S1 , B7X , B7h.5 , PRO1291 , VCTN1
Database
Research Area
REFERENCE
There are currently no references for Human B7-H4 protein, human IgG1 Fc tag (active) (GTX65697-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human B7-H4 protein, human IgG1 Fc tag (active) (GTX65697-pro). Be the first to share your experience with this product.